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Compara??o do Teste Quick de Intoler?ncia ? Lactose em bi?psias... » Isaúde
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Acta Cirurgica Brasileira
2013-02-05 08:17:53

Compara??o do Teste Quick de Intoler?ncia ? Lactose em bi?psias duodenais de pacientes disp?pticos com polimorfismo de nucleot?deo ?nico LCT-13910C>T associado com hipolactasia prim?ria/lactase persistente

Descrição: PURPOSE: To analyze the usefulness of Quick Lactose Intolerance Test in relation to the genetic test based on LCT-13910C>T genotypes, previously validated for clinical practice, for primary hypolactasia/lactase-persistence diagnosis. METHODS: Thirty-two dyspeptic patients that underwent upper gastrointestinal endoscopy entered the study. Two postbulbar duodenal biopsies were taken for the Quick test, and gastric antral biopsy for DNA extraction and LCT-13910C>T polymorphism analysis. DNA was also extracted from biopsies after being used in the Quick Test that was kept frozen until extraction. RESULTS: Nine patients with lactase-persistence genotype (LCT-13910CT or LCT-13910TT) had normolactasia, eleven patients with hypolactasia genotype (LCT-13910CC) had severe hypolactasia, and among twelve with mild hypolactasia, except for one that had LCT-13910CT genotype, all the others had hypolactasia genotype. The agreement between genetic test and quick test was high (p

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