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Detection limits of Legionella pneumophila in environmental samples... » Isaúde
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BMC microbiology [electronic resource]
2013-02-27 03:07:39

Detection limits of Legionella pneumophila in environmental samples after co-culture with Acanthamoeba polyphaga

Descrição: Background:The efficiency of recovery and the detection limit of Legionella after co-culture with Acanthamoeba polyphaga are not known and so far no investigations have been carried out to determine the efficiency of the recovery of Legionella spp. by co-culture and compare it with that of conventional culturing methods. This study aimed to assess the detection limits of co-culture compared to culture for Legionella pneumophila in compost and air samples. Compost and air samples were spiked with known concentrations of L. pneumophila. Direct culturing and co-culture with amoebae were used in parallel to isolate L. pneumophila and recovery standard curves for both methods were produced for each sample.Results:The co-culture proved to be more sensitive than the reference method, detecting 102-103-L. pneumophila cells in 1?g of spiked compost or 1?m3 of spiked air, as compared to 105-106 cells in 1?g of spiked compost and 1?m3 of spiked air.Conclusions:Co-culture with amoebae is a useful, sensitive and reliable technique to enrich L. pneumophila in environmental samples that contain only low amounts of bacterial cells.

Identificador: doi:10.1186/1471-2180-13-49
Volume: 0
Página: 4 a


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