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Shewanella oneidensis Hfq promotes exponential phase growth,... » Isaúde
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BMC microbiology [electronic resource]
2013-02-09 02:07:21

Shewanella oneidensis Hfq promotes exponential phase growth, stationary phase culture density, and cell survival

Descrição: Background:Hfq is an RNA chaperone protein that has been broadly implicated in sRNA function in bacteria. Here we describe the construction and characterization of a null allele of the gene that encodes the RNA chaperone Hfq in Shewanella oneidensis strain MR-1, a dissimilatory metal reducing bacterium.Results:Loss of hfq in S. oneidensis results in a variety of mutant phenotypes, all of which are fully complemented by addition of a plasmid-borne copy of the wild type hfq gene. Aerobic cultures of the hfq- mutant grow more slowly through exponential phase than wild type cultures, and hfq- cultures reach a terminal cell density in stationary phase that is ~2/3 of that observed in wild type cultures. We have observed a similar growth phenotype when the hfq- mutant is cultured under anaerobic conditions with fumarate as the terminal electron acceptor, and we have found that the hfq- mutant is defective in Cr(VI) reduction. Finally, the hfq- mutant exhibits a striking loss of colony forming units in extended stationary phase and is highly sensitive to oxidative stress induced by H2O2 or methyl viologen (paraquat).Conclusions:The hfq mutant in S. oneidensis exhibits pleiotropic phenotypes, including a defect in metal reduction. Our results also suggest that hfq mutant phenotypes in S. oneidensis may be at least partially due to increased sensitivity to oxidative stress.

Identificador: doi:10.1186/1471-2180-13-33
Volume: 0
Página: 3 a


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