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Staphylococcus aureus Autoinducer-2 quorum sensing decreases biofilm... » Isaúde
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BMC microbiology [electronic resource]
2012-12-06 02:07:20

Staphylococcus aureus Autoinducer-2 quorum sensing decreases biofilm formation in an icaR-dependent manner

Descrição: Background:Staphylococcus aureus is an important pathogen that causes biofilm-associated infection in humans. Autoinducer 2 (AI-2), a quorum-sensing (QS) signal for interspecies communication, has a wide range of regulatory functions in both Gram-positive and Gram-negative bacteria, but its exact role in biofilm formation in S. aureus remains unclear.Results:Here we demonstrate that mutation of the AI-2 synthase gene luxS in S. aureus RN6390B results in increased biofilm formation compared with the wild-type strain under static, flowing and anaerobic conditions and in a mouse model. Addition of the chemical synthetic AI-2 precursor in the luxS mutation strain (DeltaluxS) restored the wild-type phenotype. Real-time RT-PCR analysis showed that AI-2 activated the transcription of icaR, a repressor of the ica operon, and subsequently a decreased level of icaA transcription, which was presumably the major cause of the observed increased biofilm formation of the DeltaluxS strain. Furthermore, we compared the roles of the agr-mediated QS system and the LuxS/AI-2 QS system in the regulation of biofilm formation using the DeltaluxS strain, RN6911 and the Deltaagr DeltaluxS strain. Our data indicates a cumulative effect of the two QS systems on the regulation of biofilm formation in S. aureus.Conclusion:These findings demonstrate that AI-2 can decrease biofilm formation in S. aureus via an icaR-activation pathway. This study may provide clues for therapy in S. aureus biofilm-associated infection.

Identificador: doi:10.1186/1471-2180-12-288
Volume: 0
Página: 2 a


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