A PHP Error was encountered

Severity: Warning

Message: Creating default object from empty value

Filename: models/publicacao_item.php

Line Number: 20

Extracytoplasmic function (ECF) sigma factor sigmaF is involved in... » Isaúde
  Pesquisar Publicações Científicas  
  Especialidade: carregando especialidades...  Carregando...
Nome da revista:   Volume:   Número:
BMC microbiology [electronic resource]
2012-09-18 09:26:18

Extracytoplasmic function (ECF) sigma factor sigmaF is involved in Caulobacter crescentus response to heavy metal stress

Descrição: Background:The alpha-proteobacterium Caulobacter crescentus inhabits low-nutrient environments and can tolerate certain levels of heavy metals in these sites. It has been reported that C. crescentus responds to exposure to various heavy metals by altering the expression of a large number of genes.Results:In this work, we show that the ECF sigma factor sigmaF is one of the regulatory proteins involved in the control of the transcriptional response to chromium and cadmium. Microarray experiments indicate that sigmaF controls eight genes during chromium stress, most of which were previously described as induced by heavy metals. Surprisingly, sigmaF itself is not strongly auto-regulated under metal stress conditions. Interestingly, sigmaF-dependent genes are not induced in the presence of agents that generate reactive oxygen species. Promoter analyses revealed that a conserved sigmaF-dependent sequence is located upstream of all genes of the sigmaF regulon. In addition, we show that the second gene in the sigF operon acts as a negative regulator of sigmaF function, and the encoded protein has been named NrsF (Negative regulator of sigma F). Substitution of two conserved cysteine residues (C131 and C181) in NrsF affects its ability to maintain the expression of sigmaF-dependent genes at basal levels. Furthermore, we show that sigmaF is released into the cytoplasm during chromium stress and in cells carrying point mutations in both conserved cysteines of the protein NrsF.Conclusion:A possible mechanism for induction of the sigmaF-dependent genes by chromium and cadmium is the inactivation of the putative anti-sigma factor NrsF, leading to the release of sigmaF to bind RNA polymerase core and drive transcription of its regulon.

Identificador: doi:10.1186/1471-2180-12-210
Volume: 0
Página: 2 a

Mais informações

  • Twitter iSaúde
Jornal Informe Saúde

Indique o portal
Fechar [X]
  • Você está indicando a notícia: http://www.isaude.net
  • Para que seu amigo(a) receba esta indicação preencha os dados abaixo:

RSS notícias do portal  iSaúde.net
Receba o newsletter do portal  iSaúde.net
Indique o portal iSaúde.net
Notícias do  iSaúde.net em seu blog ou site.
Receba notícias com assunto de seu interesse.
© 2000-2011 www.isaude.net Todos os direitos reservados.