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Expression of sialic acids and other nonulosonic acids in Leptospira » Isaúde
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BMC microbiology [electronic resource]
2012-08-02 03:32:46

Expression of sialic acids and other nonulosonic acids in Leptospira

Descrição: Sialic acids are negatively charged nine carbon backbone sugars expressed on mammalian cell surfaces. Sialic acids are part of a larger family of nonulosonic acid (NulO) molecules that includes pseudaminic and legionaminic acids. Microbial expression of sialic acids and other nonulosonic acids has been shown to contribute to host-microbe interactions in a variety of contexts, including participation in colonization, immune subversion, and behaviors such as biofilm formation, autoagglutination, and motility. Previous research has suggested that some spirochetes may also express these molecules. Here we use a combination of molecular tools to investigate the presence of NulO biosynthetic gene clusters among clinical and environmental isolates of the genus Leptospira. Polymerase chain reaction and southern blotting suggested that a variety of leptospires encoded NulO biosynthetic pathways. High performance liquid chromatography and mass spectrometry analyses provided further biochemical evidence that spirochetes of this genus endogenously express NulO molecules, including sialic acid. Lectin-based affinity purification of NulO-modified molecules, followed by mass spectrometric identification suggests post-translational modification of surface lipoproteins, including Loa22. These findings have implications for immune evasion during systemic leptospirosis.

Identificador: doi:10.1186/1471-2180-12-161
Volume: 0
Página: 1 a


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