A PHP Error was encountered

Severity: Warning

Message: Creating default object from empty value

Filename: models/publicacao_item.php

Line Number: 20

Comparative molecular analyses of Borrelia burgdorferi sensu stricto... » Isaúde
  Pesquisar Publicações Científicas  
  Especialidade: carregando especialidades...  Carregando...
Nome da revista:   Volume:   Número:
BMC microbiology [electronic resource]
2012-07-31 03:30:40

Comparative molecular analyses of Borrelia burgdorferi sensu stricto strains B31 and N40D10/E9 and determination of their pathogenicity

Descrição: Background:Lyme disease in the United States is caused primarily by B. burgdorferi sensu stricto whileother species are also prevalent in Europe. Genetic techniques have identified severalchromosomal and plasmid-borne regulatory and virulence factors involved in Lymepathogenesis. B31 and N40 are two widely studied strains of B. burgdorferi, which belong totwo different 16 S-23 S rRNA spacer types (RST) and outer surface protein C (OspC) allelicgroups. However, the presence of several known virulence factors in N40 has not beeninvestigated. This is the first comprehensive study that compared these two strains both invitro and using the mouse model of infection.Results:Phylogenetic analyses predict B31 to be more infectious. However, our studies here indicatethat N40D10/E9 is more infectious than the B31 strain at lower doses of inoculation in thesusceptible C3H mice. Based-upon a careful analyses of known adhesins of these strains, it ispredicted that the absence of a known fibronectin-glycosaminoglycan binding adhesin,bbk32, in the N40 strain could at least partially be responsible for reduction in its binding toVero cells in vitro. Nevertheless, this difference does not affect the infectivity of N40D10/E9strain. The genes encoding known regulatory and virulence factors critical for pathogenesiswere detected in both strains. Differences in the protein profiles of these B. burgdorferi strains in vitro suggest that the novel, differentially expressed molecules may affectinfectivity of B. burgdorferi. Further exacerbation of these molecular differences in vivocould affect the pathogenesis of spirochete strains.Conclusion:Based upon the studies here, it can be predicted that N40D10/E9 disseminated infection atlower doses may be enhanced by its lower binding to epithelial cells at the site of inoculationdue to the absence of BBK32. We suggest that complete molecular analyses of virulencefactors followed by their evaluation using the mouse infection model should form the basis ofdetermining infectivity and pathogenicity of different strains rather than simple phylogeneticgroup analyses. This study further emphasizes a need to investigate multiple invasive strainsof B. burgdorferi to fully appreciate the pathogenic mechanisms that contribute to Lymedisease manifestations.

Identificador: doi:10.1186/1471-2180-12-157
Volume: 0
Página: 1 a


Mais informações

  • Twitter iSaúde
publicidade
Jornal Informe Saúde

Indique o portal
Fechar [X]
  • Você está indicando a notícia: http://www.isaude.net
  • Para que seu amigo(a) receba esta indicação preencha os dados abaixo:

RSS notícias do portal  iSaúde.net
Receba o newsletter do portal  iSaúde.net
Indique o portal iSaúde.net
Notícias do  iSaúde.net em seu blog ou site.
Receba notícias com assunto de seu interesse.
© 2000-2011 www.isaude.net Todos os direitos reservados.