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Complete genome sequence of Enterococcus faecium strain TX16 and... » Isaúde
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BMC microbiology [electronic resource]
2012-07-08 09:26:50

Complete genome sequence of Enterococcus faecium strain TX16 and comparative genomic analysis of Enterococcus faecium genomes

Descrição: Background:Enterococci are among the leading causes of hospital-acquired infections in the United Statesand Europe, with Enterococcus faecalis and Enterococcus faecium being the two mostcommon species isolated from enterococcal infections. In the last decade, the proportion ofenterococcal infections caused by E. faecium has steadily increased compared to otherEnterococcus species. Although the underlying mechanism for the gradual replacement of E.faecalis by E. faecium in the hospital environment is not yet understood, many studies usinggenotyping and phylogenetic analysis have shown the emergence of a globally dispersedpolyclonal subclusters of E. faecium strains in clinical environments. Systematic study of themolecular epidemiology and pathogenesis of E. faecium has been hindered by the lack ofclosed, complete E. faecium genomes that can be used as references.Results:In this study, we report the complete genome sequence of the E. faecium strain TX16, alsoknown as DO, which belongs to multilocus sequence type (ST) 18, and was the first E.faecium strain ever sequenced. Whole genome comparison of the TX16 genome with 21 E.faecium draft genomes confirmed that most clinical, outbreak, and hospital-associated (HA)strains (including STs 16, 17, 18, and 78), in addition to strains of non-hospital origin, groupin the same clade (referred to as the HA clade) and are evolutionally considerably moreclosely related to each other by phylogenetic and gene content similarity analyses than toisolates in the community-associated (CA) clade with approximately a 3-4% averagenucleotide sequence difference between the two clades at the core genome level. Our studyalso revealed that many genomic loci in the TX16 genome are unique to the HA clade. 380ORFs in TX16 are HA-clade specific and antibiotic resistance genes are enriched in HAcladestrains. Mobile elements such as IS16 and transposons were also found almostexclusively in HA strains, as previously reported.Conclusions:Our findings along with other studies show that HA clonal lineages harbor specific geneticelements as well as sequence differences in the core genome which may confer selectionadvantages over the more heterogeneous enterococcal CA E. faecium isolates. Which of thesedifferences are important for the success of specific E. faecium HA lineages in the hospitalenvironment remain(s) to be determined.

Identificador: doi:10.1186/1471-2180-12-135
Volume: 0
Página: 1 a

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