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Biochemical characterization of an anti-Candidafactor produced by... » Isaúde
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BMC microbiology [electronic resource]
2012-07-04 09:31:30

Biochemical characterization of an anti-Candidafactor produced by Enterococcus faecalis

Descrição: Background:Because Candida albicans is resistant to several antifungal antibiotics, there is a need toidentify other less toxic natural products, particularly antimicrobial proteins, peptides orbacteriocin like inhibitory substances. An attempt has been made to purify and characterisean anti-Candida compound produced by Enterococcus faecalis.Results:An anti-Candida protein (ACP) produced by E. faecalis active against 8 C. albicans strainswas characterised and partially purified. The ACP showed a broad-spectrum activity againstmultidrug resistant C. albicans MTCC 183, MTCC 7315, MTCC 3958, NCIM 3557, NCIM3471 and DI. It was completely inactivated by treatment with proteinase K and partially bypronase E.The ACP retained biological stability after heat-treatment at 90 degreesC for 20 min, maintainedactivity over a pH range 6-10, and remained active after treatment with alpha-amylase, lipase,organic solvents, and detergents. The antimicrobial activity of the E. faecalis strain was foundexclusively in the extracellular filtrate produced in the late logarithmic growth phase. Thehighest activity (1600 AU mL-1) against C. albicans MTCC 183 was recorded at 48 h ofincubation, and activity decreased thereafter. The peptide showed very lowhaemagglutination and hemolytic activities against human red blood cells. The antimicrobialsubstance was purified by salt-fractionation and chromatography.Partially purified ACP had a molecular weight of approximately 43 KDa in tricine-PAGEanalysis. The 12 amino acid N terminal sequence was obtained by Edman degradation. Thepeptide was de novo sequenced by ESI-MS, and the deduced combined sequence whencompared to other bacteriocins and antimicrobial peptide had no significant sequencesimilarity.Conclusions:The inhibitory activity of the test strain is due to the synthesis of an antimicrobial protein. Toour knowledge, this is the first report on the isolation of a promising non-haemolytic anti-Candida protein from E. faecalis that might be used to treat candidiasis especially inimmunocompromised patients.

Identificador: doi:10.1186/1471-2180-12-132
Volume: 0
Página: 1 a


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