A PHP Error was encountered

Severity: Warning

Message: Creating default object from empty value

Filename: models/publicacao_item.php

Line Number: 20

A comparative analysis of the heterotrimeric G-protein G-alpha,... » Isaúde
  Pesquisar Publicações Científicas  
  Especialidade: carregando especialidades...  Carregando...
Nome da revista:   Volume:   Número:
BMC microbiology [electronic resource]
2012-07-04 03:33:16

A comparative analysis of the heterotrimeric G-protein G-alpha, G-beta and G-gamma subunits in the wheat pathogen Stagonospora nodorum

Descrição: Background:It has been well established that the Galpha subunit of the heterotrimeric G-protein in the wheatpathogen Stagonospora nodorum is required for a variety of phenotypes includingpathogenicity, melanisation and asexual differentiation. The roles though of the Ggamma and Gbetasubunits though were unclear. The objective of this study was to identify and understand therole of these subunits and assess their requirement for pathogenicity and development.Results:G-protein Ggamma and Gbeta subunits, named Gga1 and Gba1 respectively, were identified in theStagonospora nodorum genome by comparative analysis with known fungal orthologues. Areverse genetics technique was used to study the role of these and revealed that the mutantstrains displayed altered in vitro growth including a differential response to a variety ofexogenous carbon sources. Pathogenicity assays showed that Stagonospora nodorum strainslacking Gba1 were essentially non-pathogenic whilst Gga1-impaired strains displayedsignificantly slower growth in planta. Subsequent sporulation assays showed that like thepreviously described Galpha subunit mutants, both Gba1 and Gga1 were required for asexual sporulation with neither mutant strain being able to differentiate either pycnidia norpycnidiospores under normal growth conditions. Continued incubation at 4degreesC was found tocomplement the mutation in each of the G-protein subunits with nearly wild-type levels ofpycnidia recovered.Conclusion:This study provides further evidence on the significance of cAMP-dependent signaltransduction for many aspects of fungal development and pathogenicity. The observation thatcold temperatures can complement the G-protein sporulation defect now provides an idealtool by which asexual differentiation can now be dissected.

Identificador: doi:10.1186/1471-2180-12-131
Volume: 0
Página: 1 a

Mais informações

  • Twitter iSaúde
Jornal Informe Saúde

Indique o portal
Fechar [X]
  • Você está indicando a notícia: http://www.isaude.net
  • Para que seu amigo(a) receba esta indicação preencha os dados abaixo:

RSS notícias do portal  iSaúde.net
Receba o newsletter do portal  iSaúde.net
Indique o portal iSaúde.net
Notícias do  iSaúde.net em seu blog ou site.
Receba notícias com assunto de seu interesse.
© 2000-2011 www.isaude.net Todos os direitos reservados.