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Analysis of a Clostridium difficile PCR ribotype 078100 kilobase... » Isaúde
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BMC microbiology [electronic resource]
2012-07-03 03:28:10

Analysis of a Clostridium difficile PCR ribotype 078100 kilobase island reveals the presence of a noveltransposon, Tn6164

Descrição: Background:Clostridium difficile is the main cause of antibiotic associated diarrhea. In the past decade, thenumber of C. difficile patients has increased dramatically, coinciding with the emergence oftwo PCR ribotypes, 027 and 078. PCR ribotype 078 is also frequently found during C.difficile outbreaks in pigfarms. Previously, the genome of the PCR ribotype 078 strain M120,a human isolate, was described to contain a unique insert of 100 kilobases.Results:Analysis of this insert revealed over 90 open reading frames, encoding proteins originatingfrom transposons, phages and plasmids. The insert was shown to be a transposon (Tn6164),as evidenced by the presence of an excised and circularised molecule, containing the ligated5\'and 3\'ends of the insert. Transfer of the element could not be shown through filter-matingexperiments. Whole genome sequencing of PCR ribotype 078 strain 31618, isolated from adiarrheic piglet, showed that Tn6164 was not present in this strain. To test the prevalence ofTn6164, a collection of 231 Clostridium difficile PCR ribotype 078 isolates from human (n =173) and porcine (n = 58) origin was tested for the presence of this element by PCR. Thetransposon was present in 9 human, tetracycline resistant isolates, originating from variouscountries in Europe, and none of the pig strains. Nine other strains, also tetracycline resistanthuman isolates, contained half of the transposon, suggesting multiple insertion steps yieldingthe full Tn6164. Other PCR ribotypes (n = 66) were all negative for the presence of thetransposon. Multi locus variable tandem repeat analysis revealed genetic relatedness amongtransposon containing isolates. Although the element contained several potential antibioticresistance genes, it did not yield a readily distinguishable phenotype.Conclusions:Tn6164 is a newly described transposon, occurring sporadically in C. difficile PCR ribotype078 strains. Although no transfer of the element could be shown, we hypothesize that theelement could serve as a reservoir of antibiotic resistance genes for other bacteria. Furtherresearch is needed to investigate the transfer capabilities of the element and to substantiatethe possible role of Tn6164 as a source of antibiotic resistance genes for other gut pathogens.

Identificador: doi:10.1186/1471-2180-12-130
Volume: 0
Página: 1 a

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