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Variation of chemosensory receptor content of Campylobacter jejuni... » Isaúde
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BMC microbiology [electronic resource]
2012-06-30 03:27:48

Variation of chemosensory receptor content of Campylobacter jejuni strains and modulation of receptor gene expression under different in vivo and in vitro growth conditions.

Descrição: Background:Chemotaxis is crucial for the colonisation/infection of hosts with C. jejuni. Central tochemotaxis are the group A chemotaxis genes that are responsible for sensing the externalenvironment. The distribution of group A chemoreceptor genes, as found in the C. jejunisequenced strains, tlp1-4, 7, 10 and 11 were determined in 33 clinical human and avianisolates.Results:Group A tlp gene content varied among the strains with genes encoding tlp1 (aspartatereceptor, ccaA) and tlp7 present in all strains tested, where as tlp11 was present in only one ofour international collection clinical isolate, C. jejuni 520, but was more prevalent (9/13) inthe freshly isolated clinical stains from patients who required hospitalisation due to C. jejuniinfection (GCH1-17). Relative expression levels of the group A tlp genes were alsodetermined in C. jejuni reference strains NCTC 11168-GS, 11168-O and 81116 using cellsgrown in vitro at 37?C, 42?C and maintained at room temperature and with cells isolateddirectly from murine and avian hosts by immune magnetic separation without subsequentculture. Gene expression of tlp genes was varied based on strain, growth conditions and invivo isolation source. Tlp1, although the most conserved, showed the lowest and most variedmRNA expression and protein production under laboratory conditions. Tlp7 was highlyexpressed at most conditions tested, and gene expression was not influenced by the tlp7 geneencoding a full length protein or one expressed as separate periplasmic and cytoplasmicdomains.Conclusion:We have shown that chemosensory receptor set variation exists among C. jejuni strains, but isnot dependent on the isolation source.

Identificador: doi:10.1186/1471-2180-12-128
Volume: 0
Página: 1 a


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