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Transcriptional analysis of the Escherichia coliColV-Ia plasmid pS88... » Isaúde
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BMC microbiology [electronic resource]
2012-06-22 03:34:59

Transcriptional analysis of the Escherichia coliColV-Ia plasmid pS88 during growth in humanserum and urine

Descrição: Background:The sequenced O45:K1:H7 Escherichia coli meningitis strain S88 harbors a large virulenceplasmid. To identify possible genetic determinants of pS88 virulence, we examined thetranscriptomes of 88 plasmidic ORFs corresponding to known and putative virulence genes,and 35 ORFs of unknown function.Results:Quantification of plasmidic transcripts was obtained by quantitative real-time reversetranscription of extracted RNA, normalized on three housekeeping genes. The transcriptomeof E. coli strain S88 grown in human serum and urine ex vivo were compared to that obtainedduring growth in Luria Bertani broth, with and without iron depletion. We also analyzed thetranscriptome of a pS88-like plasmid recovered from a neonate with urinary tract infection.The transcriptome obtained after ex vivo growth in serum and urine was very similar to thoseobtained in iron-depleted LB broth. Genes encoding iron acquisition systems were stronglyupregulated. ShiF and ORF 123, two ORFs encoding protein with hypothetical function andphysically linked to aerobactin and salmochelin loci, respectively, were also highly expressedin iron-depleted conditions and may correspond to ancillary iron acquisition genes. FourORFs were induced ex vivo, independently of the iron concentration. Other putative virulencegenes such as iss, etsC, ompTp and hlyF were not upregulated in any of the conditions studied. Transcriptome analysis of the pS88-like plasmid recovered in vivo showed a similarpattern of induction but at much higher levels.Conclusion:We identify new pS88 genes potentially involved in the growth of E. coli meningitis strainS88 in human serum and urine.

Identificador: doi:10.1186/1471-2180-12-115
Volume: 0
Página: 1 a

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