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Proteins other than the locus of enterocyte effacement-encoded... » Isaúde
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BMC microbiology [electronic resource]
2012-06-12 09:35:41

Proteins other than the locus of enterocyte effacement-encoded proteins contribute to

Descrição: Background:In this study, we present evidence that proteins encoded by the Locus of EnterocyteEffacement (LEE), considered critical for Escherichia coli O157 (O157) adherence tofollicle-associated epithelial (FAE) cells at the bovine recto-anal junction (RAJ), do notappear to contribute to O157 adherence to squamous epithelial (RSE) cells also constitutingthis primary site of O157 colonization in cattle.Results:Antisera targeting intimin-gamma, the primary O157 adhesin, and other essential LEE proteinsfailed to block O157 adherence to RSE cells, when this pathogen was grown in DMEM, aculture medium that enhances expression of LEE proteins. In addition, RSE adherence of aDMEM-grown-O157 mutant lacking the intimin protein was comparable to that seen with itswild-type parent O157 strain grown in the same media. These adherence patterns were incomplete contrast to that observed with HEp-2 cells (the adherence to which is mediated byintimin-gamma), assayed under same conditions. This suggested that proteins other than intimin-gammathat contribute to adherence to RSE cells are expressed by this pathogen during growth inDMEM. To identify such proteins, we defined the proteome of DMEM-grown-O157(DMEM-proteome). GeLC-MS/MS revealed that the O157 DMEM-proteome comprised 684proteins including several components of the cattle and human O157 immunome, orthologsof adhesins, hypothetical secreted and outer membrane proteins, in addition to the knownvirulence and LEE proteins. Bioinformatics-based analysis of the components of the O157DMEM proteome revealed several new O157-specific proteins with adhesin potential.Conclusion:Proteins other than LEE and intimin-gamma proteins are involved in O157 adherence to RSE cellsat the bovine RAJ. Such proteins, with adhesin potential, are expressed by this humanpathogen during growth in DMEM. Ongoing experiments to evaluate their role in RSEadherence should provide both valuable insights into the O157-RSE interactions and newtargets for more efficacious anti-adhesion O157 vaccines.

Identificador: doi:10.1186/1471-2180-12-103
Volume: 0
Página: 1 a

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