A PHP Error was encountered

Severity: Warning

Message: Creating default object from empty value

Filename: models/publicacao_item.php

Line Number: 20

Multi-locus variable number tandem repeat analysisof 7th pandemic... » Isaúde
  Pesquisar Publicações Científicas  
  Especialidade: carregando especialidades...  Carregando...
Nome da revista:   Volume:   Número:
BMC microbiology [electronic resource]
2012-05-25 03:31:29

Multi-locus variable number tandem repeat analysisof 7th pandemic Vibrio cholerae

Descrição: Background:Seven pandemics of cholera have been recorded since 1817, with the current and ongoingpandemic affecting almost every continent. Cholera remains endemic in developing countriesand is still a significant public health issue. In this study we use multilocus variable numberof tandem repeats (VNTRs) analysis (MLVA) to discriminate between isolates of the 7thpandemic clone of Vibrio cholerae.Results:MLVA of six VNTRs selected from previously published data distinguished 66 V. choleraeisolates collected between 1961-1999 into 60 unique MLVA profiles. Only 4 MLVA profilesconsisted of more than 2 isolates. The discriminatory power was 0.995. Phylogenetic analysisshowed that, except for the closely related profiles, the relationships derived from MLVAprofiles were in conflict with that inferred from Single Nucleotide Polymorphism (SNP)typing. The six SNP groups share consensus VNTR patterns and two SNP groups containedisolates which differed by only one VNTR locus.Conclusions:MLVA is highly discriminatory in differentiating 7th pandemic V. cholerae isolates andMLVA data was most useful in resolving the genetic relationships among isolates withingroups previously defined by SNPs. Thus MLVA is best used in conjunction with SNP typing in order to best determine the evolutionary relationships among the 7th pandemic V.cholerae isolates and for longer term epidemiological typing.

Identificador: doi:10.1186/1471-2180-12-82
Volume: 0
Página: 8 a

Mais informações

  • Twitter iSaúde
Jornal Informe Saúde

Indique o portal
Fechar [X]
  • Você está indicando a notícia: http://www.isaude.net
  • Para que seu amigo(a) receba esta indicação preencha os dados abaixo:

RSS notícias do portal  iSaúde.net
Receba o newsletter do portal  iSaúde.net
Indique o portal iSaúde.net
Notícias do  iSaúde.net em seu blog ou site.
Receba notícias com assunto de seu interesse.
© 2000-2011 www.isaude.net Todos os direitos reservados.