A PHP Error was encountered

Severity: Warning

Message: Creating default object from empty value

Filename: models/publicacao_item.php

Line Number: 20

Exploring the plant-associated bacterial communities in Medicago... » Isaúde
  Pesquisar Publicações Científicas  
  Especialidade: carregando especialidades...  Carregando...
Nome da revista:   Volume:   Número:
BMC microbiology [electronic resource]
2012-05-21 03:28:59

Exploring the plant-associated bacterial communities in Medicago sativa L

Descrição: Background:Plant-associated bacterial communities caught the attention of several investigators whichstudy the relationships between plants and soil and the potential application of selectedbacterial species in crop improvement and protection. Medicago sativa L. is a legume crop ofhigh economic importance as forage in temperate areas and one of the most popular modelplants for investigations on the symbiosis with nitrogen fixing rhizobia (mainly belonging tothe alphaproteobacterial species Sinorhizobium meliloti). However, despite its importance, nostudies have been carried out looking at the total bacterial community associated with the plant. In this work we explored for the first time the total bacterial community associatedwith M. sativa plants grown in mesocosms conditions, looking at a wide taxonomic spectrum,from the class to the single species (S. meliloti) level.Results:Results, obtained by using Terminal-Restriction Fragment Length Polymorphism (T-RFLP)analysis, quantitative PCR and sequencing of 16 S rRNA gene libraries, showed a hightaxonomic diversity as well as a dominance by members of the class Alphaproteobacteria inplant tissues. Within Alphaproteobacteria the families Sphingomonadaceae andMethylobacteriaceae were abundant inside plant tissues, while soil Alphaproteobacteria wererepresented by the families of Hyphomicrobiaceae, Methylocystaceae, Bradyirhizobiaceaeand Caulobacteraceae. At the single species level, we were able to detect the presence of S.meliloti populations in aerial tissues, nodules and soil. An analysis of population diversity onnodules and soil showed a relatively low sharing of haplotypes (30-40%) between the twoenvironments and between replicate mesocosms, suggesting drift as main force shaping S.meliloti population at least in this system.Conclusions:In this work we shed some light on the bacterial communities associated with M. sativaplants, showing that Alphaproteobacteria may constitute an important part of biodiversity inthis system, which includes also the well known symbiont S. meliloti. Interestingly, this lastspecies was also found in plant aerial part, by applying cultivation-independent protocols, anda genetic diversity analysis suggested that population structure could be strongly influencedby random drift.

Identificador: doi:10.1186/1471-2180-12-78
Volume: 0
Página: 7 a

Mais informações

  • Twitter iSaúde
Jornal Informe Saúde

Indique o portal
Fechar [X]
  • Você está indicando a notícia: http://www.isaude.net
  • Para que seu amigo(a) receba esta indicação preencha os dados abaixo:

RSS notícias do portal  iSaúde.net
Receba o newsletter do portal  iSaúde.net
Indique o portal iSaúde.net
Notícias do  iSaúde.net em seu blog ou site.
Receba notícias com assunto de seu interesse.
© 2000-2011 www.isaude.net Todos os direitos reservados.