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Genotypic characterization and safety assessment of lactic acid... » Isaúde
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BMC microbiology [electronic resource]
2012-05-17 09:30:40

Genotypic characterization and safety assessment of lactic acid bacteria from indigenous African fermented food products

Descrição: Background:Indigenous fermented food products play an essential role in the diet of millions of Africans.Lactic acid bacteria (LAB) are among the predominant microbial species in Africanindigenous fermented food products and are used for different applications in the food andbiotechnology industries. Numerous studies have described antimicrobial susceptibilityprofiles of LAB from different parts of the world. However, there is limited information onantimicrobial resistance profiles of LAB from Africa. The aim of this study was tocharacterize 33 LAB previously isolated from three different African indigenous fermentedfood products using (GTG)5-based rep-PCR, sequencing of the 16 S rRNA gene and speciesspecific PCR techniques for differentiation of closely related species and further evaluatetheir antibiotic resistance profiles by the broth microdilution method and their haemolyticactivity on sheep blood agar plates as indicators of safety traits among these bacteria.Results:Using molecular biology based methods and selected phenotypic tests such as catalasereaction, CO2 production from glucose, colonies and cells morphology, the isolates wereidentified as Lactobacillus delbrueckii, Lactobacillus fermentum, Lactobacillus ghanensis,Lactobacillus plantarum, Lactobacillus salivarius, Leuconostoc pseudomesenteroides, Pediococcus acidilactici, Pediococcus pentosaceus and Weissella confusa. The bacteria weresusceptible to ampicillin, chloramphenicol, clindamycin and erythromycin but resistant tovancomycin, kanamycin and streptomycin. Variable sensitivity profiles to tetracycline andgentamycin was observed among the isolates with Lb. plantarum, Lb. salivarius, W. confusa(except strain SK9-5) and Lb. fermentum strains being susceptible to tetracycline whereasPediococcus strains and Lb. ghanensis strains were resistant. For gentamicin, Leuc.pseudomesenteroides, Lb. ghanensis and Ped. acidilactici strains were resistant to 64 mg/Lwhereas some W. confusa and Lb. plantarum strains had a MIC value of 16 mg/L and 32mg/L respectively. No beta-haemolytic activity was observed, however, alpha-haemolytic activitywas observed in 27% (9) of the strains comprising Lb. salivarius (6), W. confusa (2) and Lb.delbrueckii (1) isolates.Conclusions:The resistance to kanamycin and vancomycin is probably an intrinsic feature since similarobservations were reported in the literature for LAB. Low prevalence of pathogenicityindicator traits were observed among the isolates especially with the presence of poorhaemolytic activities and they could therefore be considered as interesting candidates forselection of starter cultures or probiotics for different applications.

Identificador: doi:10.1186/1471-2180-12-75
Volume: 0
Página: 7 a

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