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Alternative sigma factor sigmaH activates competence gene expression... » Isaúde
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BMC microbiology [electronic resource]
0000-00-00 00:00:00

Alternative sigma factor sigmaH activates competence gene expression in Lactobacillus sakei

Descrição: Background:Alternative sigma factors trigger various adaptive responses. Lactobacillus sakei, a non-sporulating meat-borne bacterium, carries an alternative sigma factor seemingly orthologous to sigmaH of Bacillus subtilis, best known for its contribution to the initiation of a large starvation response ultimately leading to sporulation. As the role of sigmaH-like factors has been little studied in non-sporulating bacteria, we investigated the function of sigmaH in L. sakei.Results:Transcription of sigH coding for sigmaH was hardly affected by entry into stationary phase in our laboratory conditions. Twenty-five genes potentially regulated by sigmaH in L. sakei 23 K were revealed by genome-wide transcriptomic profiling of sigH overexpression and/or quantitative PCR analysis. More than half of them are involved in the synthesis of a DNA uptake machinery linked to genetic competence, and in DNA metabolism; however, sigmaH overproduction did not allow detectable genetic transformation. sigmaH was found to be conserved in the L. sakei species.Conclusion:Our results are indicative of the existence of a genetic competence state activated by sigmaH in L. sakei, and sustain the hypothesis that sigmaH-like factors in non sporulating Firmicutes share this common function with the well-known ComX of naturally transformable streptococci.

Identificador: doi:10.1186/1471-2180-12-32
Volume: 0
Página: 3 a


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